FAQ: What types of samples/materials are compatible with the Q5® Blood Direct 2X Master Mix?

This kit has been evaluated using up to 40% (v/v) human whole blood preserved with Na EDTA, K2 EDTA, Na citrate and Na heparin anticoagulants. However, we suggest starting with 5-10% (v/v) blood.

The Master Mix has also been tested with punches of dried human blood spotted on Whatman® 903 and FTA papers. We recommend starting with a single 0.5 mm punch in a 20 µL reaction or a single 1 mm punch in a 50 µL reaction.