FAQ: What concentration of whole blood is recommended for use with Q5® Blood Direct 2X Master Mix?

5-10% (v/v) whole blood is the recommended starting concentration for Q5 Blood Direct 2X Master Mix. It is possible to use up to 40% blood but cycling conditions may need to be modified. For higher blood volumes ( ≥ 20%) a 50 µL reaction volume is recommended due to difficulties in recovery of the aqueous phase from blood cell debris that remains after PCR