FAQ: Can a different bacterial strain be used with the Ph.D.™ Phage Display systems?

In theory other F+ strains containing the supE suppressor mutation (such as XL1-Blue and DH5αF´) should work with NEB’s phage display systems. However, we have not tested these strains with our libraries and do not know whether there will be any subtle effects on the expression or transport of certain peptides out of the cell. Since NEB’s Ph.D. libraries are made in E. coli K12 ER2738 (NEB #E4104S), we know that all the peptides in the libraries can be successfully expressed in this strain. Therefore, we recommend this strain over any other one.