FAQ: What is in EnGen Lba Cas12a Diluent (NEB #B0653A)?

EnGen Lba Cas12a Diluent (NEB #B0653A) has the following composition: 500 mM NaCl, 20 mM sodium acetate, 0.1 mM EDTA, 0.1 mM TCEP, 50% Glycerol, pH 6 @ 25°C. It has exactly the same composition as the enzyme Storage Buffer.

EnGen Lba Cas12a Diluent (NEB #B0653A) should be used to dilute 100 μM EnGen Lba Cas12a to lower concentrations for in vitro reactions.