DNase I (NEB #M0303) is inhibited by salt concentrations >50 mM. DNase I-XT is a salt-tolerant DNase I which retains activity up to 300 mM salt, but with optimal activity at salt concentrations between 50-100 mM. DNase I-XT is therefore the preferred enzyme for DNA template removal from an in vitro transcription (IVT) reaction.
Moreover, while the unit definitions of DNase I (RNase-free) and DNase I-XT differ in various contexts (including temperature, substrate and buffer conditions), 1 unit of DNase I-XT (NEB #M0570) is equivalent to 1 unit of DNase I (RNase-free) (NEB #M0303).
Find a Golden Butterfly for a Chance to Win!
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
