FAQ: Can components from other HiScribe™ kits, T7 RNA Polymerase (NEB #M0251) or RNAPol Reaction Buffer (NEB #B9012) be substituted in this kit?

No. The formulation of the components in this kit have been optimized for in vitro transcription using 5 mM CTP, 5 mM GTP, 5 mM UTP, 6 mM ATP and 4 mM CleanCap® Reagent AG in a 20 µl reaction.