HomeFAQsShould I chill the NEBExpress®E. coli Lysis Reagent prior to use?
FAQ: Should I chill the NEBExpress®E. coli Lysis Reagent prior to use?
No, protein extraction is most efficient when the reagent is between 15 and 30°C (room temperature). Chilling the reagent prior to use will cause the detergents to precipitate, thereby resulting in incomplete protein extraction.