HomeFAQsMy lysate is viscous; how can I resolve this?
FAQ: My lysate is viscous; how can I resolve this?
A viscous lysate results from an abundance of DNA released by the Lysis Reagent. Shear the DNA by passing the lysate through a 20-gauge needle via syringe several times. Alternatively, adding DNase I (NEB# M0303) at 10 to 100 U/mL, after addition of the Lysis Reagent will reduce the viscosity. It’s recommended to perform a small-scale lysis test with the Lysis Reagent to determine whether DNase I is necessary followed by scale up. NEB’s DNase I is fused to maltose-binding protein (MBP), making its removal via amylose resin (NEB# E8021), amylose resin high flow (NEB# E8022), or amylose magnetic beads (NEB# E8035) simple and effective.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
