FAQ: Does NEBExpress® E. coli Lysis Reagent lyse gram-positive bacterial cells or yeast cells?

NEBExpress E. coli Lysis Reagent lyses some gram-positive bacteria such as certain Bacillus strains (B. subtilis, B. thuringiensis). Addition of lysozyme and DNase I (NEB #M0303) is highly recommended to obtain optimal lysis of gram-positive bacteria. NEBExpress® E. coli Lysis Reagent has limited activity on yeast cells.