Home FAQs How do I quantify my PCR-free libraries?

FAQ: How do I quantify my PCR-free libraries?

Only qPCR-based methods (such as the NEBNext Library Quant Kit for Illumina (NEB # E7630)) can be used for accurate quantitation of PCR-free libraries. Other methods will quantify fragments that may not have adaptors at both ends, and therefore will not generate clusters or sequence.