Home FAQs Can I use the NEBNext Ultra II DNA PCR-free Library Prep Kit to prepare libraries for sequencing on Ion Torrent sequencing instruments?

FAQ: Can I use the NEBNext Ultra II DNA PCR-free Library Prep Kit to prepare libraries for sequencing on Ion Torrent sequencing instruments?

No, the adaptors required for Ion Torrent sequencing have blunt ends, rather than 5´-T overhangs and are not compatible with the NEBNext Ultra II DNA PCR-free workflow.