Home FAQs Can Type IIS restriction enzymes from other companies be used with NEBridge Ligase Master Mix?

FAQ: Can Type IIS restriction enzymes from other companies be used with NEBridge Ligase Master Mix?

Type IIS restriction enzymes from other companies, which have different specifications, have not been tested with NEBridge Ligase Master Mix.  Usage recommendations provided are made after extensive optimization.  Using non-NEB enzymes is not recommended nor supported. Please follow the reaction conditions found on the product protocol page.