HomeFAQsCan I assemble PCR amplicons instead of pre-cloned inserts using Golden Gate Assembly protocols and products?
FAQ: Can I assemble PCR amplicons instead of pre-cloned inserts using Golden Gate Assembly protocols and products?
Yes. For single-insert cloning, you can use unpurified amplicons but please expect reduced performance compared to purified amplicons. For assembly of multiple inserts, amplicons should be purified. For robust purification of amplicons from PCR reaction components, we recommend the Monarch® PCR and DNA Cleanup Kit (NEB #T1030).