Home FAQs Can I use the LunaScript Multiplex One-Step RT-PCR Kit to amplify long amplicons? 

FAQ: Can I use the LunaScript Multiplex One-Step RT-PCR Kit to amplify long amplicons? 

The LunaScript Multiplex One-Step RT-PCR Kit is optimized for multiplex RT-PCR targeting amplicons between 100 – 1500 bp and can routinely amplify amplicons up to 3 kb in single-plex reactions. For long amplicons, please choose the OneTaq® One-Step RT-PCR kit (NEB #E5315) or two-step RT-PCR. For two-step RT-PCR, use the cDNA Synthesis Selection Chart to identify the RT and DNA Polymerase best suited for your application.