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HomeFAQsWhat are the advantages of using standard LAMP (NEB #E1700, M1708, E1708) over pH-based colorimetric LAMP (NEB #M1800, M1804)?
FAQ: What are the advantages of using standard LAMP (NEB #E1700, M1708, E1708) over pH-based colorimetric LAMP (NEB #M1800, M1804)?
Our pH-based colorimetric LAMP master mixes (NEB #M1800, NEB #M1804) utilize a weakly buffered solution to allow for visual detection using a pH-sensitive dye. This simple visual readout can be particularly useful for point of need testing. However, the low buffering capacity required to trigger the pink to yellow color change limits sample compatibility, as highly buffered sample inputs or acid samples may impact the color change. The master mixes in our standard LAMP products (NEB #E1700, NEB #M1708, NEB #E1708) can more readily tolerate these types of sample inputs. These products are also compatible with non-pH-based colorimetric detection with compounds such as hydroxynaphthol blue, which results in a purple to blue color change upon amplification.