FAQ: What is the activity of Mismatch Endonuclease I in other buffers?

The relative activity of Mismatch Endonuclease I in the following buffers is indicated in the table below:
Buffer % Activity1
NEBuffer r1.1 50%
NEBuffer r2.1 100%
NEBuffer r3.1 25%
rCutSmart™ Buffer 75%
Q5® Reaction Buffer2 0%
ThermoPol Reaction Buffer2 25%
Standard Taq Reaction Buffer2 75%
Phusion HF Buffer2 100%


1 % Activity is listed in comparison to the optimal buffer, NEBuffer r2.1.

2 Supplemented with 10 mM MgCl2