HomeFAQsCan I use less of the native barcode adaptor during the ligation step (1.5.1)?
FAQ: Can I use less of the native barcode adaptor during the ligation step (1.5.1)?
If you plan to run a large number of samples, e.g. a total of 48 samples per sequencing run, you can reduce the reaction volume at step 1.5.1 by half, and also reduce the volume of every component in that reaction by half. However, if you are running 24 samples or less, we recommend following our standard protocol.