FAQ: Is O-Glycoprotease active in the presence of reducing agents or detergents?

No, O-Glycoprotease is not active in the presence of reducing agents or detergents. Addition of 1mM DTT, or detergents such as Invitrosol (Thermo, #MS1007) and Protease Max (Promega, # V2071), completely inactivate the enzyme. If using reducing agents or detergents, the glycoprotein must be purified prior to the addition of O-Glycoprotease. We recommend the detergent removal column HiPPR Detergent Removal Resin (Thermo catalog #88306), which should be used according to the manufacturer’s recommended instructions.