FAQ: Is O-Glycoprotease (IMPa) active in buffers other than 20 mM Tris-HCl pH 8.0?

Yes, the optimal pH range for O-Glycoprotease (IMPa) is pH 7.0-8.0. Enzyme activity drops significantly at pH values below 7.0 and above pH 8.0. O-Glycoprotease is 100% active in 20mM HEPES, pH 8.0.  The enzyme has a 50% reduction of activity in 15-25 mM NH4CO3 and is not active in 50 mM NH4CO3.