FAQ: How much data do I need when sequencing libraries generated using the NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies)?

In testing using samples of synthetic or heat inactivated SARS-CoV-2 RNA with Ct 30 or approximately 1,000 copies of gRNA, complete genome assembly (>90% for synthetic and >99% for heat inactivated covid19 RNA) was achieved using 250x coverage data or 24,500 reads.