HomeFAQsCan the Monarch HMW DNA Extraction Kit be used for processing yeast and/or fungal samples?
FAQ: Can the Monarch HMW DNA Extraction Kit be used for processing yeast and/or fungal samples?
We have validated our kit for processing yeast samples and have published a protocol based on working with S. cerevisiae samples. We have not internally tested the kit on filamentous fungi. However, based on our work in developing our silica kit (NEB #T3010), which uses similar lysis chemistry, we found our chemistry may work for certain fungi when the sample is frozen in liquid nitrogen and ground to a fine powder using a mortar and pestle. For mushrooms, dried starting material should be used. However, this approach would need to be tested for your specific species.