FAQ: What is the optimal heat shock time for the 96-well plate format NEB 5-alpha Competent E.coli (NEB #C3019P)?

Both the temperature and the timing of the heat shock step are important and specific to the transformation volume and vessel. Using the 96-well plate provided, 10 seconds at 42°C is optimal. Expect approximately 50% loss in transformation efficiency when heat shocking for 30 seconds.