HomeFAQsAre the Monarch HMW DNA Extraction kits compatible with samples stored in Monarch DNA/RNA Protection Reagent, RNAlater, DNA/RNA Shield etc.?
FAQ: Are the Monarch HMW DNA Extraction kits compatible with samples stored in Monarch DNA/RNA Protection Reagent, RNAlater, DNA/RNA Shield etc.?
Yes. For RNAlater®, it is sufficient to remove the stabilization solution completely before processing. Subsequently, standard protocols can be followed. If working with Monarch DNA/RNA Protection Reagent (NEB #T2011) or Zymo’s DNA/RNA Shield®, the reagent should be used as 1X dilution for lysis, rather than the lysis buffer supplied in the kit. In general, however, results obtained with fresh and frozen materials are superior over stabilized samples in terms of yield, purity and fragment length.
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