FAQ: Which one-step RT-qPCR product should I use for multiplexing?

All three products have been validated in a 5-plex reactions. For simplicity of reaction set up, and to enable the largest volume of sample input, NEB #M3019 is recommended.
For additional flexibility, the components are also available in a two-tube format (NEB #E3006), and without a ROX normalization dye (NEB #E3007).