FAQ: I have used NEBNext Custom RNA Depletion Design Tool and obtained probe sequences, what’s the next step?

The next step is to order the probes from your preferred oligo synthesis provider. Standard desalting oligo purification is sufficient for this application. No modifications at the 5′ or 3' ends are needed. Refer to the manual for the NEBNext RNA Depletion Core Reagent Set for further details.