HomeFAQsHow do I make a working probe pool to be used with the NEBNext RNA Depletion Core Reagent Set?
FAQ: How do I make a working probe pool to be used with the NEBNext RNA Depletion Core Reagent Set?
We recommend preparing an equimolar probe pool where probes are between 2-4 µM each, then using 1-3 µl of this probe pool in the probe hybridization step of the NEBNext RNA Depletion Core Reagent Set protocol. Optimization of the amount of probe used might be required for efficient depletion of your desired RNA.
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