HomeFAQsMy NTC sample(s) turned orange/yellow following incubation at 65°C. What happened?
FAQ: My NTC sample(s) turned orange/yellow following incubation at 65°C. What happened?
If the non-template control turns orange or yellow following incubation, the assay results are invalid and may indicate cross-contamination during reaction set up, potentially from the SARS-CoV-2 Positive Control. If the NTC reaction changes color in repeat testing, replace all reagent stocks and clean workspace with an acceptable surface decontaminate such as 10% bleach (1:10 dilution of commercial 5.25-6.0% sodium hypochlorite).