FAQ: Can NEBuilder® HiFi DNA Assembly master mix remove both 3′ and 5′ end mismatches? 

Yes, NEB HiFi can remove these mismatches up to 10bp from the end, allowing for the DNA ends to anneal without the overhang
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    NEBuilder® HiFi DNA Assembly Removal of 3´ end Mismatches

    Did you know that one of the advantages of NEBuilder HiFi is that it removes 3’ and 5’ -end mismatch sequences prior to fragment assembly? Learn how!