HomeFAQsCan I shorten or extend the incubation time of Sce PUS1 with target RNA?
FAQ: Can I shorten or extend the incubation time of Sce PUS1 with target RNA?
We found that the majority of Sce PUS1 targeted uridines are converted to pseudouridines after 2 hours of incubation. However, preferred target sites may be converted after shorter incubation times. Depending on the substrate, incubation for more than 2 hours can further increase the uridine to pseudouridine conversion ratio.