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HomeFAQsCan I scale up or scale down the reaction volume of the Sce PUS1 reaction?
FAQ: Can I scale up or scale down the reaction volume of the Sce PUS1 reaction?
We observed a drop in Sce PUS1 activity in reaction volumes smaller or larger than 100 µl. Thus, we recommend a minimum reaction volume of 100 µl, and to using multiple 100 µl reactions if scaling up for additional substrate is needed.