FAQ: What is the effect of Mg2+ concentration on Sce PUS1 activity?

For certain RNA templates we found that the absence of Mg2+ in the reaction buffer can have a stimulatory effect on Sce PUS1 activity, while it can have an inhibitory effect on others. On other RNA templates, Mg2+ may be required for efficient Sce PUS1 activity.

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Sce PUS1