FAQ: Can I substitute a different buffer and/or salt concentration in the Column Buffer?

Yes, HEPES, MOPS, and phosphate buffers (at pH values ranging from 6.5 to 8.5) can be used instead of Tris-HCl in the Column Buffer with similar results. NaCl or KCl concentrations of 25 mM to 1 M are also compatible with the affinity purification.