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HomeFAQsWhy is Golden Gate Assembly also used for single insert cloning?
FAQ: Why is Golden Gate Assembly also used for single insert cloning?
While Golden Gate is normally used for insert assemblies of 5–10 or more fragments, it also allows easy and highly efficient cloning of single inserts following the provided directions. Golden Gate can also be used with diverse single insert populations for library preparations and directed evolution requiring multiple site mutagenesis.