HomeFAQsCan you recommend a cell lysis buffer that is compatible with Lambda Protein Phosphatase (NEB #P0753)?
FAQ: Can you recommend a cell lysis buffer that is compatible with Lambda Protein Phosphatase (NEB #P0753)?
Lambda Protein Phosphatase (Lambda PP) is very sensitive to different reaction buffers. SDS at any concentration inhibits the enzyme. Ideally, the lysis buffer should have the closest composition to the optimal recommended buffer for Lambda PP, which is: 1X NEBuffer for PMP: 50 mM HEPES, 100 mM NaCl, 2 mM DTT, 0.01% Brij-35, pH 7.5, supplemented with 1mM MnCl2. These buffers are supplied with the enzyme. The ideal lysis buffer can also contain any mild non-ionic detergent such as 1% NP-40 or Triton X-100. If the PMP buffer is not ideal, another recommended lysis buffer is: 20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 1 µg/ml leupeptin, pH 7.5.