We do not recommend storing TtAgo with a guide “loaded.” While the “loaded” nucleoprotein complex should be stable for 1–2 days if stored properly at -20–4°C, the presence of ssDNA guide is detrimental to long-term stability and reliable guided-endonuclease activity. Mix TtAgo and ssDNA guide shortly before intended use and in proportion to the scale of the experiment you wish to perform. Store the complex on ice when not used immediately.
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