HomeFAQsAre genes under bacterial promoter control expressed with the NEBExpress® Cell-free E. coli Protein Synthesis System?
FAQ: Are genes under bacterial promoter control expressed with the NEBExpress® Cell-free E. coli Protein Synthesis System?
Although endogenous E. coli RNA polymerase is present in the cell lysate, there will be minimal expression of genes under bacterial promoters. Protein yields will be very low in comparison to the high protein synthesis driven by T7 RNA Polymerase.
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