FAQ: How many cells should I use for reliable detection?

The Luna Cell Ready Lysis Module has a lysis capacity of 10 - 100,000 cells in a 50 µl lysis reaction. Typically, 1-2 µl cell lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into a 20 µl downstream RT-qPCR). Most transcripts can be detected from 20 to 200 cells in a typical 20 µl reaction. However, some cell lines may contain high levels of inhibitors that affect cell lysis or the RT-qPCR reaction. For a quick check of gene expression, ~1000 cells per 50 μl reaction is a good starting point for optimization. Please see additional FAQ’s for guidance regarding specific cell lines.