FAQ: Which buffer should I use with Hi-T4 DNA Ligase?

If trying to ligate cohesive ends, we recommend using Hi-T4 DNA Ligase in 1X T4 DNA Ligase Buffer.

If trying to ligate blunt ends or single-base overhangs, Hi-T4 DNA Ligase should be used in our PEG-containing StickTogether™ DNA Ligase Buffer. Do not heat kill the reaction, as heat treating the PEG in the StickTogether™ DNA Ligase Buffer will inhibit transformation.