Home FAQs What is the difference between the unit definitions of T4 DNA Ligase (NEB #M0202) and Hi-T4 DNA Ligase (NEB #M2622)?

FAQ: What is the difference between the unit definitions of T4 DNA Ligase (NEB #M0202) and Hi-T4 DNA Ligase (NEB #M2622)?

Both T4 DNA Ligase and Hi-T4 DNA Ligase unit definitions are defined as 50% ligation of 6 μg HindIII fragments, however, the incubation temperature differs. The T4 DNA Ligase unit definition is determined at 16°C, while the Hi-T4 DNA Ligase unit is determined at 25°C.