FAQ: Which buffer should I use with Salt-T4 DNA Ligase?

If trying to ligate cohesive ends or blunt ends in the presence of >100 mM salt, we recommend using Salt-T4 DNA Ligase in 1X T4 DNA Ligase Buffer. If trying to ligate blunt ends or single-base overhangs, Salt-T4 DNA Ligase should be used in StickTogether™ DNA Ligase Buffer. We do not recommend supplementing the StickTogether™ DNA Ligase Buffer with > 100 mM salt, as PEG in the StickTogether™ DNA Ligase Buffer and DNA will precipitate, inhibiting ligation.

If trying to ligate DNA in the presence of >100 mM salt, we recommend using 1X T4 DNA Ligase Buffer. Do not heat kill the reaction, as heat treating the PEG in the StickTogether™ DNA Ligase Buffer will inhibit transformation.