HomeFAQsWhy is imidazole not necessary in the lysis/binding buffer? Why does the wash buffer contain only 5 mM imidazole?
FAQ: Why is imidazole not necessary in the lysis/binding buffer? Why does the wash buffer contain only 5 mM imidazole?
NEBExpress Ni resin utilizes a highly selective, proprietary resin that requires less imidazole throughout the purification in comparison to conventional Ni NTA based resins. The optimal concentration of imidazole will be target/impurity dependent and will therefore require optimization.
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