HomeFAQsHow can I remove imidazole from a protein sample?
FAQ: How can I remove imidazole from a protein sample?
Imidazole does not typically interfere with downstream applications and therefore removal is optional. However, imidazole does absorb UV at 280 nm which can lead to inaccurate protein quantitation. If it is necessary to eliminate the imidazole, it can be removed by dialysis, ammonium sulfate precipitation, ultrafiltration or by using a size-exclusion desalting column.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.