HomeFAQsCan I use Q5U Hot Start High-Fidelity DNA Polymerase to amplify bisulfite-treated DNA?
FAQ: Can I use Q5U Hot Start High-Fidelity DNA Polymerase to amplify bisulfite-treated DNA?
Yes. Q5U Hot Start High-Fidelity DNA Polymerase is a modified Q5 High-Fidelity DNA Polymerase which efficiently amplifies uracil-containing templates. The optimal cycling conditions for bisulfite-treated DNA are different from other applications. Please see Section 2 of the protocol for more details.
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