HomeFAQsAre the DNA fragments produced by Q5U Hot Start High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3′ overhang that Taq DNA Polymerase yields?
FAQ: Are the DNA fragments produced by Q5U Hot Start High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3′ overhang that Taq DNA Polymerase yields?
As with other Q5 product formulations, Q5U Hot Start High-Fidelity DNA Polymerase produces blunt-ended products. If TA cloning is required, 3′A-overhangs can be added with Taq DNA Polymerase or Klenow (exo-) DNA Polymerase. It is very important to remove all the Q5U Hot Start High-Fidelity DNA Polymerase first by purifying the PCR product. The proofreading activity of Q5 is very strong, so any residual polymerase will degrade the A-overhangs as they are added.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.