HomeFAQsHow can I determine if the globin mRNA and rRNA depletion was efficient?
FAQ: How can I determine if the globin mRNA and rRNA depletion was efficient?
To assess depletion efficiency, design RT qPCR primers for the sample species’ globin mRNA and rRNA, and primers for a housekeeping gene. Compare globin mRNA and rRNA content before and after depletion.
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