Yes, dsDNA Fragmentase can be heat inactivated by incubation at 65°C for 15 minutes in the presence of 50mM DTT. However, the sample must be purified for most downstream applications, even after heat denaturation. It is important to note that heating can damage the DNA. Stopping the reaction with EDTA and then removing EDTA is more gentle on the DNA and is therefore recommended.
Find a Golden Butterfly for a Chance to Win!
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
