FAQ: Is there an advantage to treating my sample with both α-Lytic Protease and Trypsin?

Yes. Site-specific cleavage after lysine and arginine residues by trypsin creates peptides that are positively charged but may be too large for mass spectrometric detection. Performing a double digest of the substrate with Trypsin and α-Lytic Protease creates smaller peptides, thereby increasing both sequence coverage and the likelihood of identifying novel post-translational modifications.