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HomeFAQsHow are EM-seq libraries prepared from cell-free DNA (cfDNA)?
FAQ: How are EM-seq libraries prepared from cell-free DNA (cfDNA)?
With cfDNA, there is no need to shear the DNA before library preparation. 10 – 200 ng of cfDNA can move directly into NEBNext Ultra II DNA library preparation, and then EM-seq enzymatic conversion and library amplification steps are followed as outlined in the product manual.