HomeFAQsWhat are to the advantages of performing purification under denaturing conditions?
FAQ: What are to the advantages of performing purification under denaturing conditions?
Purification under denaturing conditions can reduce the activity of proteolytic enzymes, reducing the chances that your target protein is degraded throughout the purification. Purification under native conditions may be hindered if the target protein is insoluble, aggregates in inclusion bodies, or possesses a tertiary structure that sequesters the His-tag.
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