FAQ: What are the recommendations for pH based elution, as opposed to imidazole?

For both imidazole and pH based purifications the cell lysis should be done in buffered solution adjusted to pH 7.4, with sodium chloride supplemented up to 0.3 M. Following binding, a wash buffer with a pH of approximately 6.3 is recommended. Elution can be carried out by utilizing a buffer with a pH between 4.5 and 5.2.